[관악장비] 형광,발광 마이크로플레이트 리더

용도 및 원리 Multi-color detection in fluorescence, luminescence, FRET & BRET. SPARK is multi-functional microplate reader. This instrument can measure 4 applications, fluorescence, luminescence, time resonance fluorescence, and fluorescence polarization. And it support the temperature control, plate shaking and sample dispensing. 주요성능 1) 광원 : Xenon Flash lamp 2) Plate format : 1well ~384well plate, Cuvettes 3) 형광 a. 파장선택 : Fusion Optic (Monochromator and Filter) b. 파장범위 : Top-Ex 230 ~ 900 nm/Em 280-900nm, Bottom-350 ~ 900 nm c. 파장 정확성 : Ex: < 1 nm ; Em: < 2 nm d. 파장 재현성 : < 1 nm e. Well scanning : 100 x 100 data points 까지 가능 f. TRF, FRET, HTRF, TR-FRET 가능 4) 편광(FP) a. 파장범위 : 300 ~ 850 nm 6) 발광 (Luminescence) a. Spectral 범위 : 370 ~ 700 nm 6) TRF a. 감도 Limit of detection F/F ≤ 40 fM (≤ 4 amol/well; 100 μl) Limit of detection F/M & M/F ≤ 65 fM (≤ 6.5 amol/well; 100 μl) Limit of detection M/M ≤ 100 fM (≤ 10 amol/well; 100 μl) 8) Filter (형광) 9) Dual Injectors (Injection in 6 ~ 384 well plates) - Syringe sizes : 1 ml or 0.5ml - Pump 속도 : 100 ~ 300 μl/sec - Injection volume : 5 ~ 1000 μl, 1 μl씩 선택 가능 - Dead volume : ≤ 100 μl 9) 측정 속도 - 96 well plate (FI) ≤ 13 sec - 384 well plate (FI) ≤ 30 sec 10) Plate shaking : Linear, Orbital 과 Double Orbital (다양한 Amplitudes포함) 11) 온도 컨트롤 : 상온에서 42℃까지