유세포 분석기
Submitted by rnd_admin on Tue, 10/22/2024 - 00:10
ename_ko:
유세포 분석기
ename_en:
Flow Cytometer
emodel:
FACS Canto II - 6 color
institution:
암연구소
location:
연건 암연구소 중앙연구지원실
epname:
하동현
tel:
02-3668-7468
email:
hdh4698@snu.ac.kr
link:
https://cri.snu.ac.kr/reservation/equipment/list
keyword:
의의
형광을 띤 살아있는 세포나 입자에 강력한 자기장을 걸어 발산되는 신호를 확인하여 세포의 특성을 분석(analysis)하는 장치
상세 규격
1. Laser : Vis. 488nm(Blue), 633nm(Red)
2. 8개 Detector
1) 488nm
2 light scatter(FSC, SSC)
6 fluorescence channels
FITC, PE, PE-TxRed, PE-Cy5, PreCP, PE-Cy7
2) 633nm
2 fluorescence channels
APC, Alexa-Fluor 700, APC-Cy7
3. Software : FACSDiva version 12.0
4. Automated Fluidics Cart
특징
- Three Lasers : 488, 633
- Main Laser Life time 9,500 ~ 10,000 Hour
- 10 Parameters : FSC, SSC, FL 6-Colors
- Flow cell with gel-coupled collection lens
- Detector arrays : Octagon, Trigon, Trigon Optics
- Automated Fluidics Cart
Sheath 20L container
Waste containers
Cleaning containers
- Fluorescence sensitivity : particle 당 100 FITC, 50 PE)
- < 0.1% System carryover
- System Automation
Spectral overlap correction
Startup, shutdown and
cleaning cycle
- Highest 18Bit data resolution
응용분야
면역학적, 세포생물학적, 종양학적 세포의 성상 관찰 및 질환 연구
code:
CRI-9998